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KMID : 0984720050370010039
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Infection and Chemotherapy
2005 Volume.37 No. 1 p.39 ~ p.46
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Molecular Epidemiological Typing of Methicillin-Resistant Staphylococcus aureus Using Random Amplified Polymorphic DNA Analysis
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Jun Hee-Jae
Kim Jung-Man
Woo Jong-Soo
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Abstract
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BACKGROUND: The random amplified polymorphic DNA (RAPD) analysis was investigated to see if this method could be a useful tool for monitoring of epidemic outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) among patients and healthcare workers (HCW) in the intensive care units (ICU).
METHODS: Thirty-eight MRSA strains were isolated from patients and HCW in Dong-A University Hospital ICU from October, 1998 to December, 1998 (10 patients and 8 HCW) and May, 2001 to July, 2001 (15 patients and 5 HCW). All strains were typed according to antimicrobial susceptibility and RAPD analysis patterns. mecA genes were detected using polymerase chain reaction (PCR).
RESULTS: Twenty one of 25 (84%) and 12 of 13 (92%) MRSA, isolated from patients and HCW, respectively, were mecA positive. mecA positive MRSA were classified into 18 different types by RAPD analysis.
CONCLUSION: DNA fingerprinting using RAPD analysis is a simple, effective, and rapid method for discriminating MRSA strains, and may be applicable in detecting outbreaks of S. aureus infections in the ICU.
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KEYWORD
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Methicillin-resistant Staphylococcus aureus, Random amplified polymorphic DNA analysis
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